Resolution and tailing factor: real acceptance criteria Writing it up because I had to work it out twice and would rather nobody else did.
Asking about resolution and tailing factor on behalf of the question I keep seeing asked badly, including by me.
Framed properly it is answerable. Framed the usual way it is not, and that is most of why the previous threads went nowhere.
Narrowing the opening post, because the general version has more than one answer.
Particle size and column dimensions determine what resolution is achievable at all. A 5 micrometre 250 millimetre column and a sub-2 micrometre 100 millimetre column are different instruments in practice.
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Coming back to: Column chemistry choices for a 40-residue peptide
On the subject in the title: Column chemistry choices for a 40-residue peptide Working notes rather than a conclusion. Column chemistry choices — I have the observation and I do not trust my interpretation of…
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2 | 43k | 16mo | |
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Area percent versus weight percent: the confusion that causes most arguments — what changed since
Area percent versus weight percent: the confusion that causes most arguments — what changed since Writing it up because I had to work it out twice and would rather nobody else did. What changes if the…
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+30 | 34 | 40k | 13mo |
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System suitability injections: if they failed, the run did not happen
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+23 | 27 | 6k | 11mo |
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Gradient steepness and apparent purity: a worked comparison
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2 | 51k | 22mo | |
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Revisiting: Theoretical plates: useful number or ritual?
Asking directly, because I could not find a straight answer: Revisiting: Theoretical plates: useful number or ritual? Asking about Theoretical plates directly, because I have read four threads on it and each…
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+16 | 21 | 1.2k | 1d |
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Second pass at: LOD and LOQ: definitions people get wrong
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+47 | 51 | 40k | 10mo |
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Follow-up: Trifluoroacetate content and its consequences
On the subject in the title: Trifluoroacetate content and its consequences Working notes rather than a conclusion. A follow-up question about Trifluoroacetate content that I did not know to ask the first…
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+12 | 16 | 5.7k | 21mo |
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Sample size in a voluntary survey: the selection problem
Posting this under the heading it deserves: Sample size in a voluntary survey: the selection problem Everything below is what sits behind that. Sample size: setting out the arithmetic in full, because I have…
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+1 | 5 | 12k | 15mo |
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Contributing data without breaching anyone's privacy — what changed since
On the subject in the title: Contributing data without breaching anyone's privacy — what changed since Working notes rather than a conclusion. What changes if the standard account of Contributing data without…
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Attributing a new peak: degradation product or excipient?
Asking directly, because I could not find a straight answer: Attributing a new peak: degradation product or excipient? A narrow question about attributing a new peak, deliberately narrow, because the broad…
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2 | 15k | 18mo |