Topic summary

Why quantitation is the hard part, not detection

Analytics › Home & field testing LOD & LOQ third-party testing ELISA

This is a generated summary. It shows the 5 most-liked posts from a topic of 16, in their original order, with the accepted answer included where one exists. It is a reading aid and it will miss nuance — the full topic is the record.

h.espinozaTL230 Dec 2024#3

w.novak, post #2: Picking up the opening post: that is the part I would want checked first. Documenting home checks with dates and lots makes them accumulate into something. Undocumented ones evaporate and get remembered selectively. Flagging that the sources on this are thinner than the confidence in the thread suggests. Go to post

Source for the quantitation figure, since it was asked for. It is in the discussion rather than the abstract, which is why the version circulating is stronger than the paper is.

Reading the surrounding paragraph is worth the two minutes. The authors are more careful than their summarisers.

31 likes in reply to #2 19mo

p.dialloTL212 Mar 2025 · edited#7

No disagreement from me. Posting only so the question does not look ignored.

23 likes 17mo

z.okonkwoTL222 Jun 2025#14

Quantitation: I would want to see the raw numbers rather than the summary before agreeing. Summaries lose exactly the information that would settle this.

18 likes 13mo

system_suitabilityTL3Analytical chemist6 Jul 2025#15

Building on post #14 rather than restating it.

The reason quantitation is hard to answer is that the obvious measurement and the relevant quantity are not the same thing, and substituting one for the other is silent.

26 likes 13mo

j.moreauTL219 Jul 2025#16

y.mensah, post #6: I disagree with the framing of quantitation above, and I think it is a substantive disagreement rather than a terminological one. Setting out why, so it can be checked. The reasoning depends on an assumption that is doing a lot of work and is never stated. If the assumption holds, the conclusion follows. I do not think it holds generally. Go to post

Quantitation at home is the part that does not survive scrutiny. Precision adequate to distinguish 96 from 98 per cent requires instrumentation and calibration that a kit does not have.

That is the practical version. The rigorous version is longer and says the same thing.

19 likes in reply to #6 12mo

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Revisiting: Can a home kit substitute for a laboratory report? A careful noAnalytics › Home & field testing · 56 replies

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