The Peptide CommonsEst. May 2024
Independent. We sell nothing and are affiliated with no manufacturer or pharmacy. Every moderation action is logged in public
Analytics · HPLC & UHPLC

About the HPLC & UHPLC category

Pinned Wiki
K
KLindqvistTL4 Moderator8 Feb 2026#1
Community wiki post. Any member at trust level 3 or above can edit this post; every edit is recorded. Last edited by mira.patel on 21 May 2026.
  • 11 Mar 2026 — KLindqvist: Restructured into sections so the outline is navigable.
  • 21 May 2026 — mira.patel: Added the limitations paragraph that review asked for.
Editors: KLindqvist, mira.patel, hana.sato
Staff post. Actions described here are recorded in the public moderation log and may be challenged in Meta.

Reversed-phase separations, gradients, columns, integration, and reading a chromatogram properly.

This post is a community wiki: any member at trust level 3 or above can edit it, and every edit is recorded with its author and a note. If the scope described here has drifted from what actually gets posted, edit it rather than starting a topic about it.

What belongs here

  • Questions specific to hplc & uhplc, with the units and the context stated.
  • Reports and datasets, with the collection method stated.
  • Disagreement about the substance, conducted in public.

What belongs elsewhere

  • Anything urgent belongs in Safety, immediately, and not here.
  • Anything about how this site works belongs in Meta.
  • Requests for a maintained page belong in Wiki requests.

Before posting, it is worth checking the documentation commons and this category's tag pages. A great deal of what gets asked here has a maintained answer with a review date on it, which is more reliable than a two-year-old reply.

0 likes 6mo
BN
bench_notesTL4 Moderator6 Apr 2026#2
Staff post. Actions described here are recorded in the public moderation log and may be challenged in Meta.

Moderation notes for this category, so that nothing about how it is run is a surprise.

Topics get moved into and out of here regularly. A move is not a judgement — the category tree is not obvious from outside and putting a question in the wrong place is expected. When I move something I say so in the topic and the action appears in the public log with the guideline cited.

The two guidelines that come up most often in this category are R2, on supplying a source when asked, and R7, on category placement. Neither is used punitively.

1 like 4mo
KB
k.brandl_deTL3Translator · DE17 May 2026#3

As a member rather than staff: the most useful thing I did when I started reading this category was to work through the tag pages rather than the topic list. The topic list shows you what is recent. The tags show you what is settled.

12 likes 2mo
NC
n.cardosoTL223 Jun 2026#4

Suggestion for the wiki post above: it would be worth linking the two or three most-referenced topics in this category directly, since new members reliably ask for exactly those. Happy to make the edit myself if nobody objects, with a revision note.

25 likes 1mo
PB
p.boatengTL227 Jul 2026#5
KLindqvist, post #1: Reversed-phase separations, gradients, columns, integration, and reading a chromatogram properly. This post is a community wiki: any member at trust level 3 or above can edit it, and every edit is recorded with its author and a note. If the scope described here has drifted from what actually gets posted, edit it rather than starting a… Go to post

Thank you for taking the time. That was more work than a reply usually is.

0 likes in reply to #1 18h

Suggested topics

TopicParticipantsRepliesViewsActivity
Carryover and the ghost peak from last week's standard — what changed since
Carryover and the ghost peak from last week's standard — what changed since Writing it up because I had to work it out twice and would rather nobody else did. What changes if the standard account of Carryover…
FDLTZYFWAA+98 114 37k 22d
[2026 update] Detection wavelength and why 214 nm and 280 nm disagree
Detection wavelength and why 214 nm and 280 nm disagree — setting out what I have, and where I think it stops being reliable. I would like to disagree carefully with the settled view on Detection wavelength,…
HVKAMCDKR+69 76 920 6h
Coming back to: Column chemistry choices for a 40-residue peptide
On the subject in the title: Column chemistry choices for a 40-residue peptide Working notes rather than a conclusion. Column chemistry choices — I have the observation and I do not trust my interpretation of…
MLFPHB 2 43k 16mo
Gradient steepness and apparent purity: a worked comparison — the long version
Gradient steepness and apparent purity: a worked comparison — the long version — setting out what I have, and where I think it stops being reliable. A narrow question about Gradient steepness and apparent…
TNENDS 2 32k 1d
Theoretical plates: useful number or ritual?
Theoretical plates: useful number or ritual? I have a specific reason for asking rather than idle curiosity, and the context is below. Asking about theoretical plates directly, because I have read four…
MPALAV 2 284 9mo

Related topics — sharing the tags HPLC, peak integration, gradient method

TopicParticipantsRepliesViewsActivity
What a reversed-phase purity number actually is
The question in the title: What a reversed-phase purity number actually is I will give what I have already checked below so nobody repeats it. I would like to understand what this number means before I repeat…
NIBTDHFEF+87 101 27k 4h
Particulates in a reconstituted solution: identifying the likely source — what changed since
Particulates in a reconstituted solution: identifying the likely source — what changed since — setting out what I have, and where I think it stops being reliable. What changes if the standard account of…
GPMSCV 2 129 14mo
Area percent versus weight percent: the confusion that causes most arguments — what changed since
Area percent versus weight percent: the confusion that causes most arguments — what changed since Writing it up because I had to work it out twice and would rather nobody else did. What changes if the…
VBSADMRLEN+30 34 40k 13mo
Revisiting: Why I ask for the gradient before I discuss the number
Posting this under the heading it deserves: Revisiting: Why I ask for the gradient before I discuss the number Everything below is what sits behind that. I would like to understand what this number means…
DNIDGHECFA+15 19 43k 10mo
Follow-up: Attributing a new peak: degradation product or excipient?
Attributing a new peak: degradation product or excipient? I have a specific reason for asking rather than idle curiosity, and the context is below. A follow-up question about Attributing a new peak that I did…
KIABCSGV+15 19 671 17mo