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Compounds · Repair & healing peptides · continued

Reading a preclinical wound-healing model and its relevance to a human tendon posts 121–150

This is a continuation of a long topic, addressed by post number rather than by page. Start at post 1.

NN
n.nybergTL222 Nov 2024#121

On post #117 — agreed on the reasoning, with one qualification.

I think the reading a preclinical wound-healing model question is answerable and has not been answered, which is a more optimistic position than most of this thread.

14 likes 20mo
CL
c.lundgrenTL223 Nov 2024#122
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c.cardosoTL223 Nov 2024#123

This is the answer, and the reason it is the answer is the more useful part.

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m.lehtinenTL223 Nov 2024#124

The preclinical work is genuinely interesting and it is preclinical. A rodent tendon model is a model of a rodent tendon, and the translation record from that kind of model is poor.

0 likes 20mo
DO
dr_okonkwoTL4 Moderator23 Nov 2024#125

Nothing in this subcategory is medical advice and several members here are describing experiments on themselves. Both of those things should be stated plainly rather than implied.

9 likes 20mo
CG
c.grimaldiTL223 Nov 2024#126
Knowlton, post #70: Post #66 describes the usual case. This is about the unusual one. If someone has run reading a preclinical wound-healing model properly I would rather read that than my own reconstruction of it. Posting mine only because the thread has gone quiet. Go to post

Adding the measurement that post #125 says would settle it.

What I would tell a new member reading about reading a preclinical wound-healing model for the first time: the confident posts are not the reliable ones, and the reliable ones are longer.

2 likes in reply to #70 20mo
PW
PharmNotes_WhitfieldTL4Pharmacist23 Nov 2024#127
ms_holloway, post #42: Second this, and I would have said it less carefully. Go to post

For anyone arriving from search engines: this subcategory discusses published preclinical work and the absence of published clinical work. It does not endorse or recommend these compounds.

Take it as a starting point and not as a specification.

0 likes in reply to #42 20mo
JF
j.fonsecaTL223 Nov 2024 · edited#128

What would change the position on repair peptides: adequately powered, published, peer-reviewed randomised controlled human trials with pre-specified outcomes. That is the standard applied to every other therapeutic claim on this site and it is the standard applied here.

29 likes 20mo
FL
f.laurentTL223 Nov 2024#129
CW
cohort_watchTL2Member23 Nov 2024#130

BPC-157: the preclinical literature is extensive, mostly from a small number of research groups, and reports effects across a wide range of injury models. The breadth of reported effects is itself worth noting — a compound that improves outcomes in tendon, muscle, gut, nerve and bone through a single mechanism would be remarkable, and remarkable claims deserve proportionate scrutiny.

1 like 20mo
NZ
n.zielinskiTL224 Nov 2024#131

Right, and stated more narrowly than I would have dared to state it.

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MJayawardenaTL3Regular24 Nov 2024#132

Answering the question post #130 raises rather than the one it answers.

The number people quote for reading a preclinical wound-healing model is a central estimate presented without its interval, and the interval is wide enough that the estimate is nearly uninformative on its own.

0 likes 20mo
MB
ma.balogunTL224 Nov 2024#133

What would change the position on repair peptides: adequately powered, published, peer-reviewed randomised controlled human trials with pre-specified outcomes. That is the standard applied to every other therapeutic claim on this site and it is the standard applied here.

Marking that as an opinion rather than a finding.

1 like 20mo
D
DOdendaalTL3Regular24 Nov 2024#134
c.draganov, post #110: Taking post #107 at face value and following it one step further. The question underneath reading a preclinical wound-healing model is usually "how would I tell?" rather than "what is true?", and that one has a method attached to it. Write down what you would expect to see under each hypothesis before you collect anything. If they… Go to post

The pentadecapeptide sequence is short and stable enough to survive conditions that would degrade a larger peptide, which is one of the few things about it that is well characterised.

Reading it back, the second half matters more than the first.

6 likes in reply to #110 20mo
RI
r.ilungaTL224 Nov 2024#135
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e.dalgleishTL3Regular24 Nov 2024 · edited#136

Everything in post #134 holds. The case it does not cover is the one I have.

What I would check first on reading a preclinical wound-healing model is whether the thing being measured moved or whether the way of measuring it moved. Those look identical in a graph.

31 likes 20mo
ER
e.roosTL224 Nov 2024#137
d.szymanski, post #97: Answering the question post #95 raises rather than the one it answers. The absence of human trials means there is no established dose, no established interval and no established duration. Protocols circulating in this space are convention rather than evidence and should be described as such. Go to post

Filing a mild objection to the consensus on reading a preclinical wound-healing model. Mild because I might be wrong; an objection because nobody has addressed the case that does not fit.

0 likes in reply to #97 20mo
SG
s.grahameTL2Member24 Nov 2024#138
j.fonseca, post #128: What would change the position on repair peptides: adequately powered, published, peer-reviewed randomised controlled human trials with pre-specified outcomes. That is the standard applied to every other therapeutic claim on this site and it is the standard applied here. Go to post

Anecdote is the whole of the human evidence base here, and this community's convention is to say so rather than to aggregate anecdotes into something that sounds like data.

3 likes in reply to #128 20mo
EC
e.coelhoTL224 Nov 2024#139

The preclinical work is genuinely interesting and it is preclinical. A rodent tendon model is a model of a rodent tendon, and the translation record from that kind of model is poor.

10 likes 20mo
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f.abrahamsenTL2Member25 Nov 2024#140

Understood, and I withdraw the assumption I opened with.

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a.nascimentoTL225 Nov 2024#141
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k.brandl_deTL3Translator · DE25 Nov 2024#142
s.kuusela, post #59: Post #55 and I disagree about the size of the effect, not about the direction. Reading a preclinical wound-healing model: the model shows whether a mechanism is plausible in a specific context. It does not show magnitude of effect in humans, does not show safety profile in humans, and does not show whether the effect survives in a more… Go to post

Taking post #139 at face value and following it one step further.

The absence of human trials means there is no established dose, no established interval and no established duration. Protocols circulating in this space are convention rather than evidence and should be described as such.

I checked the source rather than the summary, and they differ.

0 likes in reply to #59 20mo
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a.teixeiraTL225 Nov 2024 · edited#143

Noted, and thank you for writing it out rather than summarising it.

26 likes 20mo
RF
resistance_firstTL2Regular25 Nov 2024#144

Dose-response is unstudied in humans for essentially everything in this family, which means the confident numbers in circulation came from somewhere other than a trial.

The uncertainty is in the assumption, not in the calculation.

13 likes 20mo
CH
c.haddadTL225 Nov 2024#145
b.dumitru, post #107: I had written a reply contradicting post #103 and deleted it. Here is what survived. Oral versus injected administration claims for BPC-157: the preclinical work studied injection. Oral claims are common in supplier marketing and poorly supported by published evidence. If you are comparing orality between compounds, that is a… Go to post

The absence of adequately powered published randomised human trials is the central fact about BPC-157. This site states that plainly rather than hedging. Mechanistic plausibility does not substitute for it.

2 likes in reply to #107 20mo
PN
plateau_notesTL2Regular25 Nov 2024#146

Oral versus injected administration claims for BPC-157: the preclinical work studied injection. Oral claims are common in supplier marketing and poorly supported by published evidence. If you are comparing orality between compounds, that is a distinction worth noting.

0 likes 20mo
NV
n.vukovicTL225 Nov 2024#147
c.ostergaard, post #28: The pentadecapeptide sequence is short and stable enough to survive conditions that would degrade a larger peptide, which is one of the few things about it that is well characterised. Go to post

Answering the question post #144 raises rather than the one it answers.

Two claims get bundled together under reading a preclinical wound-healing model and they need separating. The descriptive one — this is what was observed — is usually well supported. The causal one — this is why — usually is not.

Almost every disagreement in threads like this one dissolves once you say which of the two you are making.

19 likes in reply to #28 20mo
AD
appeals_deskTL3Regular25 Nov 2024#148

The arithmetic in post #147 is right; the assumption feeding it is the part to check.

On reading a preclinical wound-healing model, the part that usually goes wrong is that the question is asked as though it has one answer. It has a range, and the width of the range is the interesting bit.

If you can post the two or three numbers you are working from, several people here will check the arithmetic rather than argue about the conclusion.

8 likes 20mo
YR
y.rahimiTL226 Nov 2024#149

The pentadecapeptide sequence is short and stable enough to survive conditions that would degrade a larger peptide, which is one of the few things about it that is well characterised.

Two sources, same conclusion, and I could not rule out that one copied the other.

12 likes 20mo
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preregisteredTL326 Nov 2024#150