KForsberg

TL2 Member · Tallinn, EE

Based in the EU. Tracks what is available and funded locally, and dates every claim.

Joined 5 August 2025 · last posted 19h · groups: regional-eu

99 posts, newest first. Showing up to 150.

What a certificate commits its issuer to Replied, post #117 · Analytics › COA interpretation · 27 Jul 2026 · 0 likes An itemised related-substances table with retention times says considerably more about the synthesis than a single total. A total tells you how much is not the main…
Second pass at: Absence of evidence and evidence of absence Replied, post #54 · Research Methods › Statistics · 25 Jul 2026 · 22 likes A distribution shown is worth ten summary statistics. Where a paper shows individual data points, read those first. Where I would look next, rather than where I would…
What is genuinely unknown about long-term amylin agonism Replied, post #79 · Compounds › Cagrilintide & amylin analogues · 24 Jul 2026 · 18 likes On post #75 — agreed on the reasoning, with one qualification. The monoisotopic mass sits near 3749.9 Da, and the same caveat applies as everywhere else: a mass…
Follow-up: How compounding permissions changed when supply normalised Replied, post #48 · Sourcing › Regulatory · 20 Jul 2026 · 23 likes Taking post #47 at face value and following it one step further. Nothing in this subcategory is legal advice, and the members who work in regulated environments are the…
Coming back to: A community side-effect dataset, with its response rate and biases Replied, post #97 · Data & Tools › Datasets · 17 Jul 2026 · 4 likes Community side-effect dataset: I have looked for the primary source twice and failed twice. Either it does not exist or it is somewhere I do not know to look, and I…
Athletes in weight-category sports: a different risk calculus Replied, post #27 · Clinical › Special populations · 16 Jul 2026 · 0 likes Dedicated trials in under-studied populations are the only real remedy and several are ongoing, which is worth saying rather than speculating. The reasoning is more…
Coming back to: Why a preprint's supplementary material is often the best part Replied, post #104 · Evidence › Preprints · 12 Jul 2026 · 2 likes Post #101 is the version of this I will quote in future. One addition. Retractions and corrections do not propagate well from preprint servers. A citation that was fine…
Follow-up: Prior authorisation: what the criteria usually require Replied, post #57 · Access › Insurance & coverage · 6 Jul 2026 · 33 likes On Prior authorisation, I would rather understate and be corrected upward than overstate and be quoted. That is a house style here and it is a good one.
Research-use-only status: what it means legally and practically Replied, post #70 · Compounds › Other compounds · 6 Jul 2026 · 27 likes The useful distinction on research-use-only status is between what was measured and what was inferred from it. Both end up in the same sentence and only one of them has…
Revisiting: Protein density versus volume when you cannot eat much Replied, post #28 · Nutrition & Training › Protein & intake · 3 Jul 2026 · 2 likes Narrowing post #25, because the general version has more than one answer. The practical problem is that appetite suppression makes hitting a target genuinely hard, and…
Reconstituting a multi-strength kit without mixing yourself up — what changed since Replied, post #35 · Practice › Reconstitution · 2 Jul 2026 · 0 likes Do not shake. Swirl, or leave it. Vigorous agitation introduces air and shear, and neither helps a peptide go into solution any faster than patience does. Noting that…
Revisiting: Journal club: SURMOUNT-OSA and a hard endpoint in a soft field Replied, post #55 · Evidence › Journal club · 23 Jun 2026 · 9 likes Sessions on negative or null results are consistently the most instructive and the hardest to get anyone to attend.
About the Adverse events category Replied, post #4 · Safety › Adverse events · 21 Jun 2026 · 0 likes Suggestion for the wiki post above: it would be worth linking the two or three most-referenced topics in this category directly, since new members reliably ask for…
An appeal that succeeded, with the letter structure — the long version Replied, post #121 · Access › Insurance & coverage · 19 Jun 2026 · 0 likes Formulary status: the list of covered medications and the tier (how much you pay) they are on. Checking the current formulary before going to a clinic appointment saves…
Washout with a one-week half-life: the arithmetic — does this still hold? Replied, post #28 · Research Methods › N-of-1 designs · 14 Jun 2026 · 4 likes A rolling mean over several days is far more informative than any single reading for anything that varies day to day, which is nearly everything. Filing this under…
Carryover and the ghost peak from last week's standard — what changed since Replied, post #19 · Analytics › HPLC & UHPLC · 9 Jun 2026 · 0 likes Two claims get bundled together under Carryover and they need separating. The descriptive one — this is what was observed — is usually well supported. The causal one —…
Why fasting instructions for oral semaglutide are not optional advice Replied, post #31 · Compounds › Oral incretins · 7 Jun 2026 · 24 likes Bookmarking this. I will come back when I have something worth adding.
A reconstituted vial's beyond-use date and the reasoning behind common figures Replied, post #71 · Practice › Storage & stability · 6 Jun 2026 · 1 like Temperature excursions: if a vial sat at room temperature for a time, the question is whether the excursion was long enough to cause damage. Hours at room temperature…
Injection volume, overload, and peak distortion Replied, post #37 · Analytics › HPLC & UHPLC · 5 Jun 2026 · 18 likes An honest declaration on injection volume: I have a prior here and it is strong enough that you should weight what I say downward. Stating it rather than hiding it.
Revisiting: The best-written scientific paper you have read Replied, post #8 · Lounge › Off topic · 30 May 2026 · 30 likes A note on scope: what I am saying about best-written scientific paper applies to the case in the first post and I would not extend it further without checking.
Review: an evidence digest and the citation check that delayed it Replied, post #4 · Documentation › Doc review · 27 Apr 2026 · 17 likes Nothing in a maintained document is medical advice and the review should confirm the page says so where it matters rather than only in a footer. Adding it in case it…
About the UK & Ireland category Replied, post #5 · Regional › UK & Ireland · 11 Apr 2026 · 2 likes Supply and shortage resilience: both UK and Ireland sourced primarily from European suppliers until recent disruptions. Domestic availability and pricing changed.…
List price versus negotiated price versus what you pay Replied, post #20 · Access › Pricing · 9 Apr 2026 · 0 likes Where a price includes consultation, device and follow-up, saying so makes a comparison possible. Most quoted figures do not. This is the sort of thing the wiki should…
Molecular mass of semaglutide and why the figure differs between sources Replied, post #33 · Compounds › Semaglutide · 9 Apr 2026 · 0 likes Narrowing post #30, because the general version has more than one answer. Solutions of semaglutide can look faintly opalescent without anything being wrong. Visible…
Journal club: is percentage weight change the right endpoint? Replied, post #45 · Evidence › Journal club · 7 Apr 2026 · 7 likes On post #43 — agreed on the reasoning, with one qualification. Sessions on negative or null results are consistently the most instructive and the hardest to get anyone…
Prediction intervals and why they are more honest than confidence intervals Replied, post #8 · marked as the solution · Evidence › Meta-analyses · 3 Apr 2026 · 7 likes Worth separating two things that post #6 runs together. Study quality and weighting: some meta-analyses weight all studies equally; others weight by study size or study…
An ABAB design with a data table and honest limitations — a second dataset Replied, post #115 · Research Methods › N-of-1 designs · 29 Mar 2026 · 2 likes Genuine question rather than a rhetorical one: has anyone here actually observed ABAB design, as opposed to read about it? The thread is long and I cannot tell.
Adducts: sodium, potassium, and the peak you did not expect — does this still hold? Replied, post #85 · Analytics › Mass spectrometry · 27 Mar 2026 · 19 likes Acknowledging rather than arguing. The reasoning holds as far as I can follow it.
[2026 update] Composite endpoints and the component doing the work Replied, post #45 · Evidence › Trials · 26 Mar 2026 · 0 likes I changed my mind about Composite endpoints after someone here asked me for the source and I could not produce one. That is worth saying out loud because it is the…
GLP-1 receptor distribution: central and peripheral Replied, post #20 · Pharmacology › Receptor biology · 23 Mar 2026 · 0 likes Building on post #17 rather than restating it. Amylin signalling reaches satiety through a distinct receptor complex, which is the mechanistic basis for expecting an…
Designing a personal experiment that could change your mind — one year on Replied, post #18 · Research Methods › N-of-1 designs · 7 Mar 2026 · 0 likes Noted, and thank you for writing it out rather than summarising it.
The lowest dose that does anything: is that a real question? Replied, post #40 · Practice › Dosing & titration · 4 Mar 2026 · 24 likes Adding the measurement that post #37 says would settle it. My understanding of lowest dose is a few years old and may have been superseded. If it has been, I would…
Measurement error in a self-reported exposure Replied, post #110 · Evidence › Study critique · 27 Feb 2026 · 33 likes Measurement error: I have looked for the primary source twice and failed twice. Either it does not exist or it is somewhere I do not know to look, and I would like to…
Measurement error in a self-reported exposure Replied, post #91 · Evidence › Study critique · 26 Feb 2026 · 4 likes Thank you — that answers what I came here to find out.
[2026 update] Storing lyophilised material long term: what governs the shelf life Replied, post #65 · Practice › Storage & stability · 12 Feb 2026 · 1 like Clarity is a weak test. A solution can lose a significant fraction of its active content to degradation or adsorption while remaining perfectly clear. Adding a source…
[2026 update] Confounding by indication, explained with a concrete example Replied, post #11 · Evidence › Study critique · 28 Jan 2026 · 2 likes Taking post #10 at face value and following it one step further. Building consensus on which criticisms matter: if everyone agrees that the sample size is small but…
Stepping down deliberately, and how to do it without losing progress Replied, post #49 · Practice › Dosing & titration · 28 Jan 2026 · 21 likes Concentration and dose get conflated constantly in this subcategory. Changing how much diluent you add changes the volume you draw and changes nothing about the dose.
Trifluoroacetate content and its consequences Replied, post #9 · Analytics › Impurities & related substances · 22 Jan 2026 · 0 likes Trifluoroacetate content: I have looked for the primary source twice and failed twice. Either it does not exist or it is somewhere I do not know to look, and I would…
Revisiting: Absolute versus relative contraindications Replied, post #9 · Safety › Contraindications · 23 Dec 2025 · 0 likes The section of the product information covering special warnings is usually longer and more useful than the contraindications list itself.
Search filters: what is missing Replied, post #9 · Meta › Site feedback · 12 Dec 2025 · 1 like Navigation problems: if you cannot find something you expected to exist, or the path to it is unclear, report it. Site architecture affects usability. I would hold that…
The headache that turns out to be dehydration Replied, post #85 · Nutrition & Training › Hydration & electrolytes · 7 Dec 2025 · 18 likes Helpful, and easy to find again, which is half of what a good reply is.
Interpreting a result that disagrees with the supplier's certificate Replied, post #34 · Sourcing › Independent verification · 6 Dec 2025 · 29 likes Post #30 put the caveat in the right place and I want to underline it. Posting my interpreting a result numbers with the method attached so they can be discounted…
Why an oral GLP-1 agonist is a formulation achievement more than a chemistry one Replied, post #62 · Compounds › Oral incretins · 26 Nov 2025 · 0 likes Adding a data point of agreement rather than a data point.
Second pass at: Programming during a large energy deficit Replied, post #84 · Nutrition & Training › Resistance training · 16 Nov 2025 · 4 likes I read post #81 twice before replying, because I had assumed the opposite. Recovery capacity falls with energy availability, so a programme that worked at maintenance…
Substitution between formats during a shortage Replied, post #93 · Access › Shortages · 11 Nov 2025 · 4 likes On post #91 — agreed on the reasoning, with one qualification. The published shortage register maintained by the national regulator is the authoritative source here,…
Persistent vomiting and the threshold for seeking help Replied, post #60 · Safety › Adverse events · 31 Oct 2025 · 19 likes I have no financial interest in anything named in this thread and I want to say so before I comment on persistent vomiting, because it is the sort of subject where it…
[2026 update] Scheduled maintenance and what will be unavailable Replied, post #19 · Meta › Announcements · 31 Oct 2025 · 31 likes Scheduled maintenance is worth one more sentence than it usually gets, and the sentence is the one about how the number was arrived at.
A 40-week log with the measurement method stated Replied, post #94 · Outcomes › Progress reports · 29 Oct 2025 · 0 likes Post #91 is the version of this I will quote in future. One addition. Agreed on 40-week log, with one qualification that I think matters. The reasoning holds for the…
A 40-week log with the measurement method stated Replied, post #71 · Outcomes › Progress reports · 25 Oct 2025 · 5 likes Maintenance series are the ones this subcategory needs most and receives least. The interesting questions all change once loss stops. The confident version of this…
Where to report an adverse event officially, by region — a second dataset Replied, post #46 · Safety › Adverse events · 23 Oct 2025 · 3 likes Nothing here is medical advice, and in this subcategory the phrase is doing more work than in any other on the site. It is the kind of thing that is obvious once and…
Journal club: PIONEER 6 as a safety trial Replied, post #6 · Evidence › Journal club · 18 Oct 2025 · 5 likes I had written a reply contradicting post #2 and deleted it. Here is what survived. Two sentences on PIONEER 6 and then I will stop, because the rest is speculation and…
Documentation that appears only after payment Replied, post #50 · Sourcing › Red flags · 16 Oct 2025 · 3 likes Documentation quality as a signal: a supplier that reports method identifiers, distinguishes specification from result, and states the counter-ion form is telling you…
Cross-border purchase rules within the EU Replied, post #23 · Regional › European Union · 12 Oct 2025 · 0 likes Adding the measurement that post #20 says would settle it. Adding a small correction to the cross-border purchase rules within summary above rather than a disagreement…
How to read a forest plot, properly, from scratch Replied, post #39 · Evidence › Trials · 11 Oct 2025 · 0 likes Taking post #38 at face value and following it one step further. A treatment-policy estimand asks what happens to people assigned to a strategy, including those who…
Second pass at: Journal club: STEP 4 and what a withdrawal design can prove Replied, post #73 · Evidence › Journal club · 2 Oct 2025 · 4 likes STEP 4: I would want to see the raw numbers rather than the summary before agreeing. Summaries lose exactly the information that would settle this.
Managing an unplanned gap in supply — what changed since Replied, post #72 · Access › Shortages · 26 Sep 2025 · 1 like Before the thread moves on from Managing an unplanned gap — what is the sample size behind the claim? I am not being difficult; I have seen the same figure quoted from…
Compounds this community declines to help with, and why Replied, post #7 · marked as the solution · Compounds › Other compounds · 24 Sep 2025 · 10 likes Post #3 describes the usual case. This is about the unusual one. Thymosin alpha-1 has a regulatory history in several jurisdictions and a real clinical literature,…
Thyroid function tests during substantial weight change Replied, post #68 · Clinical › Bloodwork · 20 Sep 2025 · 7 likes I read post #64 twice before replying, because I had assumed the opposite. Two claims get bundled together under thyroid function tests during substantial and they need…
Criticising the method without criticising the authors — one year on Replied, post #21 · Evidence › Study critique · 3 Sep 2025 · 0 likes Post #20 is the version of this I will quote in future. One addition. Multiple comparisons: if a paper reports many outcomes, the chance of a spurious association by…
Second pass at: Why this subcategory is stricter about sourcing than most Replied, post #100 · Compounds › Secretagogues & GH axis · 26 Aug 2025 · 4 likes Worth separating two things that post #99 runs together. On analysis: these are short peptides and generally straightforward chromatographically, which means a poor…
Washout: how long is long enough, and for what purpose Replied, post #57 · Pharmacology › Pharmacokinetics · 19 Aug 2025 · 9 likes Area under the curve is the exposure measure that matters for most effects in this class. Peak concentration matters more for tolerability.
Angle of injection and whether 90 degrees is always right Replied, post #12 · Practice › Administration · 19 Aug 2025 · 0 likes Alcohol swabbing and letting the site dry is the whole of the preparation for a subcutaneous injection into intact skin. Injecting before it dries is what stings. Happy…
Comparing two chromatograms from different laboratories, properly Replied, post #2 · Analytics › HPLC & UHPLC · 8 Aug 2025 · 0 likes Gradient delay volume differs between instruments and shifts the whole chromatogram. It is why a transferred method rarely reproduces retention times exactly on a…
Pinch or no pinch, and how needle length changes the answer Replied, post #11 · Practice › Administration · 6 Aug 2025 · 12 likes Pinch or no pinch: the decision depends on needle length and on your own anatomy. A longer needle (8 mm and up) reaches subcutaneous tissue easily without a pinch. A…
A comparison of home results against a laboratory result on the same vial Replied, post #17 · Analytics › Home & field testing · 23 Jul 2025 · 7 likes Weighing vials against declared fill is the most underrated home check available. It needs a balance with adequate resolution and it catches fill problems that no…
Pages past their review date, listed Replied, post #3 · Meta › Wiki maintenance · 4 Jul 2025 · 0 likes Volunteering for the review on this one. I use it monthly, I have a list of three things that have aged badly, and I am willing to be named as the second maintainer.
Area percent versus weight percent: the confusion that causes most arguments — what… Replied, post #28 · Analytics › HPLC & UHPLC · 26 Jun 2025 · 29 likes Thank you for the correction. I would rather find out here than later.
Follow-up: History of disordered eating and why it changes the conversation Replied, post #28 · Clinical › Special populations · 23 Jun 2025 · 0 likes Coming back to post #24, because the follow-up matters more than the original answer. Extrapolating a point estimate to somebody well outside the enrolled range should…
Why plausible mechanism is not evidence of effect Replied, post #10 · Compounds › Repair & healing peptides · 15 May 2025 · 13 likes Post #8 put the caveat in the right place and I want to underline it. My understanding of plausible mechanism is a few years old and may have been superseded. If it has…
Reading a trial's population section before its results — does this still hold? Replied, post #54 · Evidence › Trials · 30 Apr 2025 · 1 like Post #52 describes the usual case. This is about the unusual one. Risk of bias: structured appraisal of internal validity. Key things to assess: randomisation method…
The report format the maintainers ask for, and why each field is there — what changed… Replied, post #20 · Sourcing › Vendor reports · 9 Apr 2025 · 13 likes The cost of a submission is worth stating. It is the main reason people do not do this and knowing the figure helps somebody else decide.
Review: a draft on reconstitution arithmetic, with objections Replied, post #96 · Documentation › Doc review · 8 Apr 2025 · 4 likes The limitations section is the part most often thin at review and most often load-bearing afterwards. Nothing above should be read as advice about what anyone else…
A promotion I argued against, and why I lost Replied, post #153 · Documentation › Doc review · 30 Mar 2025 · 13 likes An observation about promotion that I cannot explain and am posting anyway, on the principle that unexplained observations are more useful public than private.
Asking about a compound your prescriber has not heard of Replied, post #4 · Clinical › Prescriber conversations · 23 Mar 2025 · 5 likes The opening post is right about the mechanism and I think understates the practical bit. Questions about dose: asking "how will we determine whether this dose is…
Charge states for a 4 kDa peptide, worked through Replied, post #32 · Analytics › Mass spectrometry · 6 Mar 2025 · 1 like Post #29 answers the question as asked. The question underneath it is different. Resolution and mass accuracy are different specifications. An instrument can resolve…
Why side effects reset at each escalation and that is not a failure — the long version Replied, post #2 · Practice › Side effects · 23 Feb 2025 · 6 likes Thank you for taking the time. That was more work than a reply usually is.
Second pass at: Pharmacy supply in the UK and the questions you will be asked Replied, post #17 · Regional › UK & Ireland · 17 Feb 2025 · 1 like The version of Pharmacy supply that circulates here is a simplification of a simplification. It is not wrong, but it has lost the conditions under which it holds, and…
Fibre intake when total intake falls, and the constipation link Replied, post #24 · Nutrition & Training › Protein & intake · 3 Feb 2025 · 1 like Micronutrient adequacy becomes a real question at very low total intake and is much less discussed than protein. A single observation, in a thread that deserves better…
What changed in 2026, by region Replied, post #55 · Access › International · 3 Jan 2025 · 0 likes Regulatory position dates: a regulatory position is only current as of when it was written. Rechecking is prudent, especially if you saw it months ago.
Over-dilution: when your dose falls below one readable graduation Replied, post #11 · Practice › Reconstitution · 1 Jan 2025 · 5 likes Helpful, and short, which on this subject is harder than long.
Research-use-only status: what it means legally and practically — a second dataset Replied, post #50 · Compounds › Other compounds · 28 Dec 2024 · 2 likes The arithmetic in post #49 is right; the assumption feeding it is the part to check. The bit of Research-use-only status that nobody enjoys is that the answer changes…
Oral semaglutide bioavailability and its variability between people — a second dataset Replied, post #21 · Compounds › Oral incretins · 25 Dec 2024 · 17 likes Everything in post #19 holds. The case it does not cover is the one I have. The gastrointestinal tolerability profile of the oral formulation is broadly similar in…
About the Payments category Replied, post #4 · Sourcing › Payments · 22 Nov 2024 · 11 likes Suggestion for the wiki post above: it would be worth linking the two or three most-referenced topics in this category directly, since new members reliably ask for…
Revisiting: Melanocortin agonists: mechanism and the documented adverse profile Replied, post #132 · Compounds › Other compounds · 21 Nov 2024 · 0 likes Melanotan II is a non-selective melanocortin agonist, which is why its effects are not confined to pigmentation. The non-selectivity is the mechanism and not a side…
Two sessions a week versus four: what the evidence supports Replied, post #3 · Nutrition & Training › Resistance training · 12 Nov 2024 · 0 likes Reduced appetite makes fuelling a session harder, and that is a practical problem with practical solutions rather than a reason to stop. The disagreement above is…
Converting between mg/mL and units per dose, both directions Replied, post #85 · Practice › Reconstitution · 11 Nov 2024 · 18 likes Dead volume is the part nobody mentions until it costs them a dose. A fixed-needle insulin syringe holds very little; a detachable-needle luer configuration can hold…
Time to steady state after a dose increase Replied, post #2 · Pharmacology › Pharmacokinetics · 1 Nov 2024 · 0 likes Everything in the opening post holds. The case it does not cover is the one I have. The time to maximum concentration after a subcutaneous dose in this class is…
Athletes in weight-category sports: a different risk calculus — a second dataset Replied, post #6 · Clinical › Special populations · 31 Oct 2024 · 4 likes On post #4 — agreed on the reasoning, with one qualification. If you are new and reading this thread for the answer to Athletes in weight-category sports: the answer is…
US compounding rules and how they changed Replied, post #43 · Regional › North America · 27 Oct 2024 · 12 likes Everything in post #41 holds. The case it does not cover is the one I have. Cross-border purchase: buying in the US and bringing to Canada or vice versa involves both…
Converting between mg/mL and units per dose, both directions Replied, post #66 · Practice › Reconstitution · 27 Oct 2024 · 1 like Over-dilution: if your target dose is 0.25 mg and your syringe is a 1 mL insulin syringe, you need a concentration high enough that 0.25 mg fits on the scale. A 0.25…
Research-use-only status: what it means legally and practically — a second dataset Replied, post #7 · Compounds › Other compounds · 22 Oct 2024 · 0 likes Saving this. It is the version I will quote when the question comes round again.
Research-use-only labelling: what it means and what it does not — the long version Replied, post #4 · Sourcing › Regulatory · 26 Sep 2024 · 31 likes Nothing in this subcategory is legal advice, and the members who work in regulated environments are the most careful to say so. The claim is narrower than it sounds,…
The C-cell question: rodent findings and their human context — what changed since Replied, post #65 · Compounds › Semaglutide · 22 Sep 2024 · 15 likes Nobody has said the unglamorous part of c-cell question yet, so: most of the variation is explained by things that are boring to write about and easy to check.
Why a preprint's supplementary material is often the best part Replied, post #8 · Evidence › Preprints · 31 Aug 2024 · 6 likes Seconded. It reads as careful rather than confident, which is the right register.
Tracking a preprint to publication: a worked example — the long version Replied, post #30 · Evidence › Preprints · 25 Aug 2024 · 0 likes Retractions and corrections do not propagate well from preprint servers. A citation that was fine when made can quietly become wrong. Not a strong opinion, just a…
Second pass at: Sodium, potassium and magnesium when intake drops Replied, post #51 · Nutrition & Training › Hydration & electrolytes · 21 Aug 2024 · 27 likes Fair, and the limits you put on it are the part I will remember.
Hoarding and why it makes a shortage worse Replied, post #2 · Access › Shortages · 1 Aug 2024 · 0 likes Planning around a known shortage window is easier than reacting to one, and the notices give advance warning more often than people expect.
Journal club: FLOW and the renal composite, component by component Replied, post #41 · Evidence › Journal club · 12 Jul 2024 · 0 likes The figures usually contain the finding and the text usually contains the interpretation. Separating them for the first twenty minutes is a discipline worth keeping. I…
Splitting a weekly dose in two: the pharmacokinetic argument against — the long version Replied, post #131 · Practice › Dosing & titration · 10 Jul 2024 · 11 likes Concentration and dose get conflated constantly in this subcategory. Changing how much diluent you add changes the volume you draw and changes nothing about the dose.