FFaulkner

TL3 Regular · Ljubljana, SI

Laboratory background. Comfortable with chromatography, cautious about extrapolating from a single certificate.

Joined 10 October 2024 · last posted 5h · groups: analytical-chemists

120 posts, newest first. Showing up to 150.

Named, unnamed and unspecified impurities as regulatory categories — the long version Replied, post #121 · Analytics › Impurities & related substances · 28 Jul 2026 · 2 likes Post #120 answers the question as asked. The question underneath it is different. Acetate content: counter-ion content. Trifluoroacetate or acetate from the salt form…
Spreadsheets: an appreciation — what changed since Replied, post #114 · Lounge › Off topic · 27 Jul 2026 · 23 likes Reframing Spreadsheets slightly, because I think the disagreement is about the question rather than the answer. If the question is "does it happen", yes. If it is "how…
What the GIP component of tirzepatide is thought to contribute, and how confident we can… Replied, post #19 · Compounds › Tirzepatide · 26 Jul 2026 · 14 likes Worth separating GIP component of tirzepatide as a question about the compound from GIP component of tirzepatide as a question about the documentation. They get…
Designing a personal experiment that could change your mind — one year on Replied, post #77 · Research Methods › N-of-1 designs · 18 Jul 2026 · 17 likes Reporting the whole series rather than the interesting segment is the discipline that makes personal data worth reading. Selective reporting is the default without…
Revisiting: Journal club: SURMOUNT-OSA and a hard endpoint in a soft field Replied, post #107 · Evidence › Journal club · 15 Jul 2026 · 4 likes Narrowing post #106, because the general version has more than one answer. The figures usually contain the finding and the text usually contains the interpretation.…
Insurance and replacement policies, compared Replied, post #87 · Sourcing › Logistics · 14 Jul 2026 · 3 likes Reading back through, this was answered upthread and I missed it. My fault.
Muscle cramps: the ordinary causes before the exotic ones — a second dataset Replied, post #38 · Practice › Side effects · 9 Jul 2026 · 0 likes Fatigue: commonly reported, frequently multifactorial. It is worth asking whether other factors have changed (sleep, training volume, diet adequacy, hydration) before…
How much context is too much context in a first post? Replied, post #14 · Start Here › How to Ask · 5 Jul 2026 · 0 likes Worth separating two things that post #12 runs together. Disagreement-style questions: "I read this and it contradicts what I heard here, which is right?" is…
Asking a follow-up: new topic or new reply? Replied, post #38 · Start Here › How to Ask · 5 Jul 2026 · 0 likes I think the asking a follow-up question is answerable and has not been answered, which is a more optimistic position than most of this thread.
[2026 update] Travelling with an injectable: documentation and practicalities Replied, post #53 · Access › International · 3 Jul 2026 · 33 likes The most useful thing anyone can add to a thin regional thread is what the official source says and where it is, rather than what happened to them.
Why pulsatile secretion matters for interpreting secretagogue claims Replied, post #3 · Compounds › Secretagogues & GH axis · 30 Jun 2026 · 1 like Following this. I have the same question and no better information than the first post.
Revisiting: Protein density versus volume when you cannot eat much Replied, post #16 · Nutrition & Training › Protein & intake · 29 Jun 2026 · 0 likes Thank you — that answers what I came here to find out.
Insurance and replacement policies, compared Replied, post #43 · Sourcing › Logistics · 26 Jun 2026 · 0 likes Insurance and replacement policies: I have looked for the primary source twice and failed twice. Either it does not exist or it is somewhere I do not know to look, and…
Journal club: LEADER as the historical anchor Replied, post #94 · Evidence › Journal club · 24 Jun 2026 · 5 likes Post #92 describes the usual case. This is about the unusual one. Small methodological point on LEADER: repeating a measurement is cheap and resolves most of what is…
Revisiting: Why the absence of controlled human data on BPC-157 matters more than people… Started this topic · Compounds › Repair & healing peptides · 24 Jun 2026 · 28 likes Revisiting: Why the absence of controlled human data on BPC-157 matters more than people admit Writing it up because I had to work it out twice and would rather nobody…
Revisiting: Protein density versus volume when you cannot eat much Replied, post #6 · Nutrition & Training › Protein & intake · 24 Jun 2026 · 0 likes Micronutrition alongside protein: eating protein foods that are also good sources of micronutrients (iron, B vitamins, zinc) solves two problems at once. This is the…
Follow-up: Prior authorisation: what the criteria usually require Replied, post #36 · Access › Insurance & coverage · 24 Jun 2026 · 6 likes Post #32 describes the usual case. This is about the unusual one. Keep every document with dates in one place. An appeal months later is assembled from whatever you kept.
Journal club: indirect comparison between two programmes, defended and attacked Replied, post #94 · Evidence › Journal club · 22 Jun 2026 · 0 likes Two questions I would want answered before drawing anything from the indirect comparison data above: how were the cases selected, and what happened to the ones that…
Revisiting: Journal club: SURMOUNT-OSA and a hard endpoint in a soft field Replied, post #38 · Evidence › Journal club · 16 Jun 2026 · 0 likes On post #36 — agreed on the reasoning, with one qualification. What I can speak to on SURMOUNT-OSA is narrow, so I will keep it narrow rather than generalising from it.…
Injecting into an area that has become firm: why not to Replied, post #30 · Practice › Administration · 7 Jun 2026 · 1 like Drawing up with one needle and injecting with another: a reasonable practice if you have extra needles. Fresh needle for injection reduces tissue drag and can decrease…
Ultrasound and skinfolds: operator dependence in practice Replied, post #5 · Outcomes › Body composition · 3 Jun 2026 · 8 likes Bone mineral density changes with substantial weight loss are measurable and slow, and are one of the better reasons to have a baseline scan. That has been true for the…
Peak-to-trough ratio at steady state for a weekly agent Replied, post #31 · Pharmacology › Pharmacokinetics · 1 Jun 2026 · 18 likes Right — I had this wrong and I am glad to have read it before it mattered.
Documenting a supplier's response to a discrepancy Replied, post #4 · Sourcing › Vendor reports · 27 May 2026 · 5 likes On the opening post — agreed on the reasoning, with one qualification. Reports of ordinary results are more valuable than reports of remarkable ones, precisely because…
Pinch or no pinch, and how needle length changes the answer — what changed since Replied, post #32 · Practice › Administration · 25 May 2026 · 1 like Adding the measurement that post #30 says would settle it. Needle length matters less than angle for subcutaneous delivery in most people. A short needle at ninety…
Coming back to: A structured critique template this community uses Replied, post #15 · Evidence › Study critique · 20 May 2026 · 13 likes A note on scope: what I am saying about structured critique template applies to the case in the first post and I would not extend it further without checking.
Sample size intuition for a personal experiment — does this still hold? Replied, post #23 · Research Methods › Statistics · 13 May 2026 · 24 likes Distinguishing three things in the Sample size intuition discussion that keep getting used interchangeably: the observation, the proposed mechanism, and the…
Review: a draft on reconstitution arithmetic, with objections — one year on Replied, post #10 · Documentation › Doc review · 9 May 2026 · 3 likes Bookmarking this. I will come back when I have something worth adding.
Molecular mass of semaglutide and why the figure differs between sources Replied, post #52 · Compounds › Semaglutide · 8 May 2026 · 0 likes Coming back to post #50, because the follow-up matters more than the original answer. On alcohol: the labelling does not contraindicate it, but it raises gastric…
Second pass at: What a 2 ppm match establishes, and what it does not Replied, post #9 · Analytics › Mass spectrometry · 2 May 2026 · 3 likes What I want from this 2 ppm match thread is the list of things that would need to be true for the claim to hold. If we can write that list, we can check it.
Coming back to: Measurement error in a self-reported exposure Replied, post #17 · Evidence › Study critique · 25 Apr 2026 · 1 like On Measurement error, I would rather understate and be corrected upward than overstate and be quoted. That is a house style here and it is a good one.
Coming back to: Specificity, linearity, accuracy, precision, range, robustness — with… Replied, post #3 · Analytics › Method validation · 21 Apr 2026 · 8 likes Where the Specificity discussion usually stalls is that nobody wants to say "I do not know" and everyone is willing to say "it varies". Those are the same sentence with…
How to ask about something you suspect is a bad idea Replied, post #76 · Start Here › How to Ask · 13 Apr 2026 · 2 likes Post #73 and I disagree about the size of the effect, not about the direction. Tag your own topic. Two accurate tags do more for how findable it is than a long title,…
Specificity, linearity, accuracy, precision, range, robustness — with real criteria Replied, post #6 · Analytics › Method validation · 11 Apr 2026 · 20 likes Precision and repeatability: within-run and between-run variability of the method. Acceptance criterion is typically a relative standard deviation of ≤2% for area…
Revisiting: Drawing up with one needle and injecting with another Replied, post #72 · Practice › Administration · 7 Apr 2026 · 7 likes Nothing in this subcategory is medical advice and none of it is a substitute for a demonstration from somebody qualified to give one, which most prescribers will do…
Spike-and-recover accuracy for a peptide assay Replied, post #13 · Analytics › Method validation · 7 Apr 2026 · 5 likes Limits of detection and quantitation: LOD is the lowest concentration that produces a signal above background. LOQ is the lowest concentration at which the method meets…
A site rotation scheme that is easy enough to actually follow Replied, post #30 · Practice › Administration · 30 Mar 2026 · 19 likes Leaving the needle in for a couple of seconds after depressing the plunger reduces leakage at the site. It costs nothing and it is the fix for the "some came back out"…
Second pass at: The storage lookup and where its data comes from Started this topic · Data & Tools › Calculators · 26 Mar 2026 · 56 likes On the subject in the title: Second pass at: The storage lookup and where its data comes from Working notes rather than a conclusion. Two things I would like separated…
Managing an unplanned gap in supply Replied, post #55 · Access › Shortages · 9 Mar 2026 · 8 likes Post #52 is the version of this I will quote in future. One addition. Device availability and compound availability are separate supply chains and the device is…
"Greater than or equal to 99%" as a specification rather than a result Replied, post #147 · Analytics › COA interpretation · 7 Mar 2026 · 1 like A chromatogram supplied as a small image is legible for peak shape and not for baseline detail. That is enough to sanity-check an integration and not enough to…
Common supplements people ask about, assessed one at a time — a second dataset Replied, post #61 · Practice › Interactions · 3 Mar 2026 · 2 likes Research-use-only compounds have no interaction data of any kind, because interaction studies are done on medicines being developed for use in people. Adding this to…
Warfarin and altered intake: the monitoring argument — the long version Replied, post #116 · Practice › Interactions · 20 Feb 2026 · 0 likes The most defensible general position in this subcategory: identify the plausible mechanism, check whether it has been studied, and where it has not, say that rather…
MHRA statements, read directly Replied, post #90 · Regional › UK & Ireland · 16 Feb 2026 · 2 likes A referral pathway that worked eighteen months ago may not exist now. The archive keeps these posts and they are read as current.
Seven-day rolling means and why weekly readings mislead — one year on Replied, post #60 · Outcomes › Progress reports · 15 Feb 2026 · 2 likes Self-reported intake is optimistic in a consistent direction, and saying so in your own report is more credible than not.
Reading an interaction checker output critically Replied, post #109 · Practice › Interactions · 3 Feb 2026 · 30 likes Anticoagulants: no direct interaction with the compounds in this class. Weight loss and body composition changes might affect the clearance or effect of warfarin if you…
Second pass at: Fasting versus non-fasting and which tests care Replied, post #10 · Clinical › Bloodwork · 28 Jan 2026 · 26 likes Fasting state, time of day, recent exercise and hydration all move several common analytes. Standardising the conditions matters more than the interval between draws.…
Non-inferiority margins: how they are chosen and how they are abused — a second dataset Replied, post #15 · Evidence › Trials · 26 Jan 2026 · 0 likes Absolute numbers, not just relative: a 30% relative reduction tells you the ratio but not the practical magnitude. The event rate in each arm and the difference between…
Oral semaglutide bioavailability and its variability between people Replied, post #29 · Compounds › Oral incretins · 20 Jan 2026 · 4 likes PIONEER 6 was a cardiovascular safety trial for oral semaglutide, not an efficacy trial. Non-inferiority for safety was demonstrated. The point estimates favoured the…
Angle of injection and whether 90 degrees is always right Replied, post #77 · Practice › Administration · 5 Jan 2026 · 29 likes Worth separating two things that post #74 runs together. Second-hand on angle of injection, so weight it accordingly — someone whose method I trust told me this and I…
Vomiting: when it is expected and when it is a reason to stop Replied, post #94 · Practice › Side effects · 29 Dec 2025 · 25 likes Post #93 is right about the mechanism and I think understates the practical bit. Dehydration is the mechanism behind more of the secondary complaints here than people…
Reading SURMOUNT-1 without the press release Replied, post #110 · Compounds › Tirzepatide · 22 Dec 2025 · 9 likes Adding thanks rather than a view. I do not have a view worth the space.
DXA precision and the least significant change for lean mass Replied, post #77 · Outcomes › Body composition · 17 Dec 2025 · 26 likes DXA on the same machine with the same technician has a least significant change for lean mass of roughly a kilogram, sometimes more. Below that, two scans are the same…
Tracking a preprint to publication: a worked example Replied, post #44 · Evidence › Preprints · 4 Dec 2025 · 10 likes The honest position on most preprints in this field is that they are interesting and provisional, and that saying both is not fence-sitting. The variance between people…
Severe gastroparesis and why this class is a poor fit — one year on Replied, post #17 · Safety › Contraindications · 3 Dec 2025 · 22 likes Narrowing post #16, because the general version has more than one answer. Allergy to the specific compound: true allergy is rare but possible. Any prior allergic…
The headache that turns out to be dehydration Replied, post #74 · Nutrition & Training › Hydration & electrolytes · 28 Nov 2025 · 1 like I had written a reply contradicting post #71 and deleted it. Here is what survived. The documentation on headache is better than this thread and I say that as someone…
Sequence verification for an obscure compound: how it is done — what changed since Replied, post #47 · Compounds › Other compounds · 24 Nov 2025 · 28 likes AOD-9604 is a fragment of human growth hormone and has been discussed as a potential weight-loss agent based on theoretical mechanism. The fragment hypothesis — that…
Lurking for two months before posting: was that the right call? Replied, post #42 · Start Here › Welcome · 19 Nov 2025 · 0 likes Off-topic posts that do not belong here belong in the Lounge instead. You are not in trouble if a moderator moves your post — they are just routing it to the right…
Long-time reader, first-time poster Replied, post #18 · Lounge › Introductions · 16 Nov 2025 · 17 likes Acknowledging rather than arguing. The reasoning holds as far as I can follow it.
Documentation that materially improves an appeal's chances Replied, post #65 · Access › Insurance & coverage · 15 Nov 2025 · 20 likes Post #63 describes the usual case. This is about the unusual one. Documentation your clinician can provide: letters from clinicians describing why standard treatments…
Second pass at: The mass-error checker and its intended scope Replied, post #64 · Data & Tools › Calculators · 15 Nov 2025 · 0 likes Mass-error checker has a well-known answer and a correct answer, and the interesting work is establishing that they are the same. Nobody has done that here yet.
Why an oral GLP-1 agonist is a formulation achievement more than a chemistry one Replied, post #43 · Compounds › Oral incretins · 14 Nov 2025 · 0 likes Answering the question post #39 raises rather than the one it answers. The gastrointestinal tolerability profile of the oral formulation is broadly similar in character…
Specificity, linearity, accuracy, precision, range, robustness — with real criteria — a… Replied, post #37 · Analytics › Method validation · 4 Nov 2025 · 0 likes Forced degradation under acid, base, oxidation, heat and light is how specificity is actually demonstrated. The chromatograms from that work are more informative than…
Second pass at: Robustness: the parameters worth deliberately varying Replied, post #20 · Analytics › Method validation · 19 Oct 2025 · 0 likes Taking post #19 at face value and following it one step further. The most useful single question about a method: what would it fail to detect? Every method has an…
Why one laboratory's 98.4% and another's 96.1% may not contradict Replied, post #26 · Analytics › Method validation · 18 Oct 2025 · 2 likes Taking post #25 at face value and following it one step further. A stability-indicating method is one demonstrated to resolve the analyte from its degradation products,…
Reading a supplier's product description as a marketing document Replied, post #84 · Compounds › Other compounds · 15 Oct 2025 · 0 likes Where a compound has a legitimate pharmaceutical form somewhere in the world, the labelling for that form is usually the single most useful document available and is…
The headache that turns out to be dehydration Replied, post #23 · Nutrition & Training › Hydration & electrolytes · 14 Oct 2025 · 0 likes Quietly grateful for the plain phrasing. Not every thread gets that.
Managing an unplanned gap in supply — what changed since Replied, post #84 · Access › Shortages · 10 Oct 2025 · 31 likes I had written a reply contradicting post #80 and deleted it. Here is what survived. The published shortage register maintained by the national regulator is the…
The 2.5 mg starting dose is not a therapeutic dose — why that matters — a second dataset Started this topic · Compounds › Tirzepatide · 9 Oct 2025 · 28 likes Posting this under the heading it deserves: The 2.5 mg starting dose is not a therapeutic dose — why that matters — a second dataset Everything below is what sits…
Cagrilintide's dosing interval and the pharmacokinetics behind it Replied, post #56 · Compounds › Cagrilintide & amylin analogues · 3 Oct 2025 · 33 likes Reconstitution at higher concentrations is worth avoiding for aggregation-prone material. The published formulation work generally uses lower concentrations than people…
Sleep quality changes during weight loss — what changed since Replied, post #6 · Wellbeing › Sleep · 22 Sep 2025 · 21 likes Consumer sleep trackers measure movement and heart rate and infer stages. The inference is imprecise and the trend is more usable than the nightly numbers. I have…
Dizziness on standing and the electrolyte explanation — does this still hold? Replied, post #2 · Practice › Side effects · 17 Sep 2025 · 19 likes The strongest argument against my own position on Dizziness on standing, stated as well as I can state it, since nobody else has yet.
Power cut for eleven hours: how I assessed it afterwards Replied, post #56 · Practice › Storage & stability · 13 Sep 2025 · 0 likes Taking post #53 at face value and following it one step further. Clarity is a weak test. A solution can lose a significant fraction of its active content to degradation…
Reverse causation in a cohort study of weight and outcome — a second dataset Replied, post #15 · Evidence › Study critique · 12 Sep 2025 · 29 likes I think the Reverse causation question is answerable and has not been answered, which is a more optimistic position than most of this thread.
Aggregated purity results across four services, with caveats — a second dataset Replied, post #108 · Data & Tools › Datasets · 10 Sep 2025 · 25 likes This is the answer, and the reason it is the answer is the more useful part.
Follow-up: Journal club: orforglipron phase 2 and the non-peptide question Replied, post #79 · Evidence › Journal club · 5 Sep 2025 · 0 likes Worth separating two things that post #75 runs together. Sessions on negative or null results are consistently the most instructive and the hardest to get anyone to…
Coming back to: What happens when a maintainer goes quiet Replied, post #8 · Documentation › Doc review · 28 Aug 2025 · 0 likes New maintainer recruitment: if a page's maintainer is inactive and updates are needed, suggesting a new maintainer here can help transition responsibility.
Immortal time bias in a claims-database study Replied, post #29 · Evidence › Study critique · 24 Aug 2025 · 22 likes Post #28 describes the usual case. This is about the unusual one. Statistical significance and clinical importance are different and both are needed. A significant…
Revisiting: List price versus negotiated price versus what you pay Replied, post #21 · Access › Pricing · 9 Aug 2025 · 24 likes Answering the question post #20 raises rather than the one it answers. Pharmacy acquisition cost: a pharmacy pays less than the patient pays, even at insurance rates.…
Repeatability versus intermediate precision Replied, post #59 · Analytics › Method validation · 25 Jun 2025 · 11 likes Post #57 answers the question as asked. The question underneath it is different. Whatever the answer on repeatability versus intermediate precision turns out to be, the…
Area percent versus weight percent: the confusion that causes most arguments — what… Replied, post #16 · Analytics › HPLC & UHPLC · 24 Jun 2025 · 0 likes That is consistent with mine, for whatever one more account is worth.
Triple agonism: additive, synergistic, or neither? Replied, post #60 · Compounds › Retatrutide · 24 Jun 2025 · 4 likes The hepatic-steatosis rationale follows directly from glucagon receptor agonism promoting fat oxidation in the liver. Mechanistic plausibility in this field has a poor…
Why one laboratory's 98.4% and another's 96.1% may not contradict Replied, post #10 · Analytics › Method validation · 17 Jun 2025 · 3 likes Building on post #8 rather than restating it. System suitability is the ongoing evidence that a validated method is still performing. Validation is done once;…
Washout: how long is long enough, and for what purpose Replied, post #36 · Pharmacology › Pharmacokinetics · 16 Jun 2025 · 18 likes Washout after stopping takes roughly the same four to five half-lives as reaching steady state. A month after the last dose is not the same as none.
Temperature loggers worth the money Replied, post #2 · Devices › Cold chain · 16 Jun 2025 · 6 likes Adding what did not work for me on temperature loggers worth the money, since the failures never get written up and they are half the useful information.
Follow-up: Tracking intake honestly when appetite is absent Replied, post #101 · Nutrition & Training › Protein & intake · 16 Jun 2025 · 16 likes Confirming post #99 from a second method, which matters more than confirming it from a second person. Density matters more than quantity when total intake is limited.…
Revisiting: Reading a denial letter as a specification for your appeal Replied, post #15 · Access › Insurance & coverage · 14 Jun 2025 · 0 likes Thank you — that answers what I came here to find out.
Asking about a compound your prescriber has not heard of Replied, post #63 · Clinical › Prescriber conversations · 11 Jun 2025 · 3 likes Everything in post #61 holds. The case it does not cover is the one I have. Prescription logistics: confirm whether it will be filled locally, requires mail order, or…
Random versus fixed effects: choosing rather than defaulting Replied, post #2 · Evidence › Meta-analyses · 10 Jun 2025 · 1 like This follows the opening post rather than contradicting it. A request rather than an answer: could whoever has the primary source for random versus fixed effects post…
Reading a rodent study on a secretagogue without over-extrapolating Replied, post #5 · Compounds › Secretagogues & GH axis · 8 May 2025 · 14 likes An update on my earlier reading a rodent study post: the pattern held for another six weeks and then stopped, which I did not predict and cannot explain.
Research-use-only labelling: what it means and what it does not — the long version Replied, post #146 · Sourcing › Regulatory · 7 May 2025 · 8 likes Generic or biosimilar entry depends on patent and exclusivity positions that are jurisdiction-specific and are frequently misreported. Adding it in case it saves…
Charge states for a 4 kDa peptide, worked through Replied, post #128 · Analytics › Mass spectrometry · 16 Mar 2025 · 0 likes Sensible. I would want the same detail before I acted on it either.
One lot is one lot: when a report becomes a pattern — the long version Replied, post #30 · Sourcing › Vendor reports · 15 Mar 2025 · 4 likes Building on post #27 rather than restating it. The cost of a submission is worth stating. It is the main reason people do not do this and knowing the figure helps…
Obstructive sleep apnoea and a hard endpoint in this class Replied, post #33 · Clinical › Comorbidities · 14 Mar 2025 · 14 likes Where two conditions pull in opposite directions, that is a clinical judgement rather than a lookup, and it is the kind of question a forum answers worst. Second-hand,…
Charge states for a 4 kDa peptide, worked through Replied, post #44 · Analytics › Mass spectrometry · 7 Mar 2025 · 0 likes Everything in post #40 holds. The case it does not cover is the one I have. Quantitation by MS: most quantitation is done by LC-UV detection at 214 nm, not by MS,…
Review: a draft on reconstitution arithmetic, with objections Replied, post #39 · Documentation › Doc review · 28 Feb 2025 · 0 likes Reviewing your own nomination is fine and is not sufficient. Somebody else has to read it for the process to mean anything. I am not the right person to answer the…
Second pass at: How to research a compound with no human data without deceiving yourself Replied, post #25 · Compounds › Other compounds · 24 Feb 2025 · 15 likes This is the first time the answer has come with its own limits attached. Appreciated.
Second pass at: How to research a compound with no human data without deceiving yourself Replied, post #18 · Compounds › Other compounds · 2 Feb 2025 · 15 likes Post #16 and I disagree about the size of the effect, not about the direction. Anyone submitting an unusual compound for testing should tell the laboratory what it is…
What changed in 2026, by region Replied, post #81 · Access › International · 16 Jan 2025 · 0 likes Where a compound is approved under a different trade name in another market, searching the local name finds documents the English name does not.
Coming back to: Cost per delivered dose, which is the number that matters Replied, post #11 · Access › Pricing · 6 Jan 2025 · 23 likes Where I part company with post #7, and it is a narrow parting. Landed cost including shipping, fees and currency conversion is what you actually pay, and quotes that…
Journal club: STEP 4 and what a withdrawal design can prove — one year on Replied, post #21 · Evidence › Journal club · 3 Jan 2025 · 5 likes Post #17 and I disagree about the size of the effect, not about the direction. The summary that goes to the digest should say what the paper establishes, what it does…
[2026 update] Journal club: SURPASS-2 and the semaglutide 1 mg comparator Replied, post #80 · Evidence › Journal club · 3 Jan 2025 · 17 likes Marking my uncertainty on SURPASS-2 explicitly. I am confident about the direction, much less confident about the size, and not confident at all that it generalises…
Follow-up: Where the four-week escalation interval comes from, and what it is not Replied, post #137 · Practice › Dosing & titration · 30 Dec 2024 · 4 likes Small methodological point on four-week escalation interval: repeating a measurement is cheap and resolves most of what is being argued about here at no cost to anyone.
Oral semaglutide bioavailability and its variability between people — a second dataset Replied, post #51 · Compounds › Oral incretins · 26 Dec 2024 · 21 likes Small methodological point on Oral semaglutide bioavailability: repeating a measurement is cheap and resolves most of what is being argued about here at no cost to…
Revisiting: Semaglutide formulation: what is in the licensed product besides the peptide Replied, post #41 · Compounds › Semaglutide · 26 Dec 2024 · 19 likes Post #40 is the version of this I will quote in future. One addition. On formulation: the licensed product is buffered and includes a preservative in the multi-dose…
Follow-up: Tirzepatide's shorter half-life and its one practical consequence Replied, post #99 · Compounds › Tirzepatide · 20 Dec 2024 · 28 likes I came in to disagree and I am leaving without a disagreement.
Converting between mg/mL and units per dose, both directions Replied, post #137 · Practice › Reconstitution · 19 Dec 2024 · 1 like Picking up post #135: that is the part I would want checked first. Preservative effectiveness is tested against a defined microbial challenge under defined conditions.…
Oxidation and deamidation mass shifts, tabulated Replied, post #22 · Analytics › Mass spectrometry · 19 Dec 2024 · 0 likes Before the thread moves on from oxidation and deamidation mass shifts — what is the sample size behind the claim? I am not being difficult; I have seen the same figure…
Coming back to: When to suspect the method rather than the sample Replied, post #83 · Analytics › Method validation · 16 Dec 2024 · 29 likes Answering the question post #80 raises rather than the one it answers. Forced degradation studies: deliberately stress the material with acid, base, oxidant, heat,…
Coming back to: When to suspect the method rather than the sample Replied, post #62 · Analytics › Method validation · 12 Dec 2024 · 24 likes This follows post #61 rather than contradicting it. Sample stability during analysis is part of validation and is routinely ignored. A preparation that degrades in the…
Body image changing more slowly than the body Replied, post #8 · Wellbeing › Mental health · 10 Dec 2024 · 0 likes On body image, I would rather understate and be corrected upward than overstate and be quoted. That is a house style here and it is a good one.
What would change my mind about repair peptides: a specific answer — one year on Replied, post #3 · Compounds › Repair & healing peptides · 22 Oct 2024 · 2 likes Stability of BPC-157 in solution: what has been measured in published work covers specific formulations under specific conditions. Extrapolating to a reconstituted…
Second pass at: Food noise reducing and the disorientation that can follow Replied, post #31 · Wellbeing › Mental health · 14 Oct 2024 · 6 likes My position on Food noise is current rather than settled. I have revised it once already and I expect to again, so treat it accordingly.
Follow-up: A site rotation scheme that is easy enough to actually follow Replied, post #22 · Practice › Administration · 11 Sep 2024 · 6 likes Leave real distance between consecutive sites rather than moving a centimetre. Repeatedly injecting one area changes the local tissue over time and eventually changes…
Correlation in a self-tracked dataset: what it can support Replied, post #153 · Research Methods › Statistics · 26 Aug 2024 · 0 likes Time-to-event analysis handles differing follow-up in a way a simple proportion cannot, which is why event rates and Kaplan-Meier estimates can differ. I would call…
Splitting a vial for two laboratories: how to do it without ruining both — what changed… Replied, post #16 · Sourcing › Independent verification · 25 Aug 2024 · 0 likes A record with four results over sixteen months is a thin record rather than a bad one, and the honest description says which.
The C-cell question: rodent findings and their human context — what changed since Replied, post #15 · Compounds › Semaglutide · 21 Aug 2024 · 10 likes If someone has run c-cell question properly I would rather read that than my own reconstruction of it. Posting mine only because the thread has gone quiet.
High-resolution MS on aggregates and what it can see — one year on Replied, post #44 · Analytics › Mass spectrometry · 19 Aug 2024 · 16 likes Electrospray ionisation produces multiply charged ions. For a 4 kDa peptide you expect mostly 2+, 3+, and 4+ charge states. Reading an electrospray spectrum means…
Chronic kidney disease and the FLOW result Replied, post #3 · marked as the solution · Clinical › Comorbidities · 9 Aug 2024 · 23 likes Building on the opening post rather than restating it. Agreed on chronic kidney disease, with one qualification that I think matters. The reasoning holds for the case…
Request: a diff view for wiki revisions Replied, post #3 · Meta › Feature requests · 29 Jul 2024 · 0 likes Request with the use case rather than the feature. I frequently want every topic that cites a particular maintained document. At the moment I can find the document from…
What happens when a maintainer goes quiet Replied, post #8 · Documentation › Doc review · 21 Jun 2024 · 6 likes Post #7 is the version of this I will quote in future. One addition. Second maintainers matter. A single maintainer is a single point of failure and every document here…
The mass-error checker and its intended scope Replied, post #15 · Data & Tools › Calculators · 27 May 2024 · 0 likes This follows post #13 rather than contradicting it. For anyone finding this later: the short answer on mass-error checker is that it depends on one thing, and the rest…